Magnetic bead-based ELISA allows rapid, inexpensive, quantitative and scalable detection of human SARS-CoV-2 antibodies

L Huergo, K Selim, M Conzentino, E Gerhardt, A Santos, B Wagner, J Alford, N Deobald, F Pedrosa, E Souza, M Nogueira, S Raboni, D Souto, F Rego, D Zanette, M Aoki, B Fornazari, H Morales, V Borges, A Nelde, J Walz, M Becker, N Schneiderhan-Marra, U Rothbauer, R Reis, K Forchhammer, J Nardin

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Received date: 14th October 2020

Here we describe a simple chromogenic magnetic bead-based ELISA which allows rapid, inexpensive, quantitative and scalable detection of human antibodies against SARS-CoV-2 using serum, plasm or blood. We show that the method is readily adaptable to use different SARS-CoV-2 antigens. The method was validated by two independent laboratories and the performance was in the same range as obtained using classic ELISA and Luminex though requiring only a fraction of consumables, instrumentation, time to deliver results and volume of sample. The results can be visually interpreted without compromising accuracy as demonstrated by validation at a point of care unit. The magnetic bead ELISA can be implemented in high throughput formats to fast track COVID-19 cases and humoral response to future SARS-CoV-2 vaccines. 

Read in full at medRxiv.

This is an abstract of a preprint hosted on a preprint server, which is currently undergoing peer review at Scientific Reports. The findings have yet to be thoroughly evaluated, nor has a decision on ultimate publication been made. Therefore, the results reported should not be considered conclusive, and these findings should not be used to inform clinical practice, or public health policy, or be promoted as verified information.

Scientific Reports

Nature Research, Springer Nature